Tomocube’s holotomography microscopes now offer a motorized stage and custom-designed stitching software. The automated combination can be fitted to any microscope model in the company’s range and produces large field-of-view images and time-lapse videos. Featuring a step size of just 70 µm, the stage allows specimens up to 8 mm x 8 mm to be imaged in their entirety. The motorized stage is equipped with a Mark & Find capability, which enables multiple cell locations to be recorded and rapidly retuned to; and Click & Shift, which centers a defined target position in the field of view. Fast image acquisition of holotomographic images on the HT-1 and HT-2 microscopes allows 2D and 3D images to be captured at rates of 150 and 2.5 frames per second respectively across the entire 8 mm x 8 mm stage acquisition area. All the possibilities offered by a large field-of-view are now available without any sample preparation, staining or labelling. In addition, the system provides structural and chemical information from the cells at high resolution, including dry mass, cell volume, shapes of sub-cellular organelles, cytoplasmic density, surface area, and deformability.

Tomocube, Inc