Integrated DNA Technologies' (IDT) PrimeTime Ebola qPCR Assay detects Zaire ebolavirus, the species involved in the 2013-2015 West African Ebola outbreak. The assay has a PCR efficiency of 98.9%, and the use of ZEN Double-Quenched Probes lowers assay background and increases signal compared to traditional single-quenched probes in qPCR assays. The portable PCR machine, the Ubiquitome Freedom4, processes four reactions (sample plus negative, positive, and extraction controls) in approximately 1 hour, and is easily controlled via smartphone or laptop. The device eliminates the need for sample preservation and transportation to a laboratory-based testing facility, and can be brought to remote locations for use during time-sensitive, public health situations. The Freedom4 and the PrimeTime Ebola qPCR Assay are now being submitted to the FDA for emergency use authorization.