Given the importance of early diagnosis and proper antibiotic treatment of Lyme disease, the Sebia Autoimmune & Infectious Diseases (Sebia AI-ID) Borrelia MTTT algorithm is designed to improve detection of early Lyme disease. The STTT algorithm requires a second tier immunoblot, which adds subjectivity and reduces sensitivity in detecting acute Lyme disease. The algorithm eliminates the need for immunoblots, helping to improve laboratory efficiency as both tiers utilize the same ELISA method.