Early diagnosis and proper antibiotic treatment of Lyme disease is essential. The Lyme immunoassay tests by Sebia—ZEUS Borrelia MTTT—are crafted with an algorithm to improve detection of early Lyme disease by up to 30% compared to the standard two-tiered testing (STTT) algorithm. The STTT algorithm requires a second tier immunoblot, which can add subjectivity and reduce sensitivity in detecting acute Lyme disease. The Borrelia MTTT algorithm eliminates the need for immunoblots with the goal of improved laboratory efficiency, as both tiers utilize the same ELISA method.