Syzygy Biotech introduces rEVAlution qPCR Master Mix, an environmentally safe, ready-to-use hot-start qPCR mixture specifically formulated with quality components to offer superior safety, speed, and signaling capabilities. EvaGreen dye was purposely selected for this formulation because it is cell membrane impermeable, making it a non-mutagenic, non-cytotoxic PCR dye that is safe for handling and safe for aquatic life, so it can be directly disposed of down the drain. EvaGreen also helps produce signals that are stronger than the SYBR green dyes. Additionally, Syzygy FlashTaq, a chemically modified hot-start Taq DNA polymerase recognized for its rapid activation and high level of compatibility was selected for use in the formulation. The Syzygy FlashTaq enzyme in the Master Mix can be activated in just two minutes. The rEVAlution qPCR Master Mix offers good yield even with reactions as low as 10ul and the enzymes in the mix have been chemically modified (as opposed to antibody modified) to help reduce the risk of contamination.